Project information
Charakterizace MAGE proteinů: kofaktory E3 ubiquitin ligáz?

Information

This project doesn't include Faculty of Science. It includes Central European Institute of Technology. Official project website can be found on muni.cz.

Project Identification

IAA501630902

Project Period

1/2009 - 12/2011

Investor / Pogramme / Project type

Academy of Sciences of the Czech Republic

Grants of distinctly investigative character focused on the sphere of research pursued at present particularly in the ASCR

MU Faculty or unit

Central European Institute of Technology

doc. Mgr. Jan Paleček, Dr. rer. nat.
Mgr. Sreenivas Reddy Bathula, Ph.D.
Mgr. Lucie Bozděchová, Ph.D.
Marc Guérineau, Ph.D., M.Sc.
Mgr. Tomáš Klumpler, Ph.D.
doc. RNDr. Jaromír Marek, Ph.D.
prof. RNDr. Vladimír Sklenář, DrSc.
Mgr. Ondrej Šedo, Ph.D.
Mgr. Kateřina Zábrady, Ph.D.
prof. RNDr. Zbyněk Zdráhal, Dr.
prof. Mgr. Lukáš Žídek, Ph.D.

Keywords

MAGE (melanoma antigen) proteins; protein structure and interactions; RING finger proteins; E3 ubiquitin ligases; DNA repair; chromatin structure; SMC5-6 complex

Cooperating Organization

The University of Sussex

Responsible person Prof. Alan R. Lehmann

The MAGE (Melanoma antigen) family consists of 35 proteins in humans that contain a "MAGE homology domain" (MHD) but have widely differing functions. The Smc5-6 complex, which functions in DNA repair, consists of MAGEG1-Nse1 heterodimer. Taking, Nse1 is a putative RING finger E3 ubiquitin ligase, there are two other reports of MAGE proteins interacting with RING-finger proteins. In addition, our structure predictions suggest that part of MHD has similarities to cullin components of E3 ligases. We will test the hypothesis that MAGE proteins are cofactors in E3 ligase complexes. We will carry out structural analyses of the MHD fold using NMR spectroscopy and X-ray crystallography to test our structural predictions. We will mutate critical residues in MAGEG1, that we predict to interact with Nse1, to see if this specifically destroys this interaction and affects DNA repair. We will search for and analyze new RING partners interacting with MAGE proteins to find out MHD common features.

Publications

Total number of publications: 11

2015

The melanoma-associated antigen 1 (MAGEA1) protein stimulates the E3 ubiquitin-ligase activity of TRIM31 within a TRIM31-MAGEA1-NSE4 complex

KOZÁKOVÁ Lucie VONDROVÁ Lucie STEJSKAL Karel CHARALABOUS Panagoula KOLESÁR Peter LEHMANN Alan R. ULDRIJAN Stjepan SANDERSON Christopher M. ZDRÁHAL Zbyněk PALEČEK Jan

Article in Periodical

Cell Cycle, year: 2015, volume: 14, edition: 6, DOI

2013

Evolution and function of non-SMC subunits of SMC5/6 complex

KOZÁKOVÁ Lucie BEDNÁŘOVÁ Kateřina HUDSON Jessica GUÉRINEAU Marc LEHMANN Alan PALEČEK Jan

Conference abstract

Year: 2013, type: Conference abstract

2012

Analysis of the Nse3/MAGE-binding Domain of the Nse4/EID Family Proteins

GUÉRINEAU Marc KŘÍŽ Zdeněk KOZÁKOVÁ Lucie BEDNÁŘOVÁ Kateřina JANOŠ Pavel PALEČEK Jan

Article in Periodical

PLoS One, year: 2012, volume: 7, edition: 4, DOI
MAGE proteiny jako kofaktory buněčných procesů

KOZÁKOVÁ Lucie HUDSON Jessica BEDNÁŘOVÁ Kateřina GUÉRINEAU Marc PALEČEK Jan

Article in Proceedings

XII. mezioborové setkání mladých biologů, biochemiků a chemiků z Čech a SLovenska, year: 2012

2011

CHARACTERIZATION OF THE NSE4/EID BINDING TO NSE3/MAGE PROTEINS

GUÉRINEAU Marc KŘÍŽ Zdeněk KOZÁKOVÁ Lucie BEDNÁŘOVÁ Kateřina BATHULA Sreenivas Reddy HUDSON Jessica LEHMANN R. Alan PALEČEK Jan

Conference abstract

Year: 2011, type: Conference abstract
Interactions between the Nse3 and Nse4 Components of the SMC5-6 Complex Identify Evolutionarily Conserved Interactions between MAGE and EID Families

HUDSON J J R BEDNÁŘOVÁ Kateřina KOZÁKOVÁ Lucie LIAO Chunyan GUÉRINEAU Marc COLNAGHI Rita VIDOT Susanne MAREK Jaromír BATHULA Sreenivas Reddy LEHMANN Alan PALEČEK Jan

Article in Periodical

PloS ONE, year: 2011, volume: 6, edition: 2, DOI

2010

The MAGE and EID proteins affect transcription regulation of target genes

KOZÁKOVÁ Lucie BEDNÁŘOVÁ Kateřina HUDSON Jessica GUÉRINEAU Marc LIAO Chunyan COLNAGHI Rita MAREK Jaromír VIDOT Susanne LEHMANN Alan PALEČEK Jan

Conference abstract

Year: 2010, type: Conference abstract
The Nse3/MAGE and Nse4/EID proteins are involved in transcription regulation

PALEČEK Jan BEDNÁŘOVÁ Kateřina HUDSON Jessica KOZÁKOVÁ Lucie GUÉRINEAU Marc LIAO Chunyan COLNAGHI Rita MAREK Jaromír VIDOT Susanne LEHMANN Alan

Conference abstract

Year: 2010, type: Conference abstract
The NSE3/MAGE interactions with NSE4/EID proteins are evolutionarily conserved

BEDNÁŘOVÁ Kateřina KOZÁKOVÁ Lucie HUDSON Jessica GUÉRINEAU Marc KOZÁKOVÁ Lucie MAREK Jaromír GUÉRINEAU Marc LEHMAN Alan R. MAREK Jaromír PALEČEK Jan LEHMANN Alan R. PALEČEK Jan

Conference abstract

Year: 2010, type: Conference abstract

2009

Architecture and activities of Structure Maintenance of Chromosome SMC5/6 complex

PALEČEK Jan

Year: 2009, type: