Project information
Synthetic consortium of Pseudomonas putida strains for biodegradation and co-utilisation of (hemi)cellulosic polymers.
- Project Identification
- GA22-12505S
- Project Period
- 1/2022 - 12/2024
- Investor / Pogramme / Project type
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Czech Science Foundation
- Standard Projects
- MU Faculty or unit
- Faculty of Science
Pseudomonas putida KT2440 has emerged as a promising bacterial platform for lignocellulose biotechnology. It is well known for its abilities to degrade polymeric lignin, catabolise and even valorise resulting aromatic chemicals. Its biochemical network has been recently expanded toward utilisation of hemicellulosic pentose sugars and their co-assimilation with lignin aromatics and cellulosic glucose. These engineering endeavours proved the exceptional metabolic flexibility of P. putida. However, its foreseen potential to degrade and use cellulosic and hemicellulosic polymers and oligomers has not yet been explored. In this project, we aim to fill in this knowledge gap. We will adopt cutting-edge genetic engineering tools for the construction of a synthetic P. putida-based consortium capable of the parallel degradation of model (hemi)cellulosic polymers and co-utilisation of the gained oligomeric and monomeric hexose and pentose sugars. Once proven functional, the consortium will become a significant step toward consolidated bioprocessing of lignocellulosic residues by P. putida.
Sustainable Development Goals
Masaryk University is committed to the UN Sustainable Development Goals, which aim to improve the conditions and quality of life on our planet by 2030.
Publications
Total number of publications: 10
2024
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Synthetically-primed adaptation of Pseudomonas putida to a non-native substrate D-xylose
Nature Communications, year: 2024, volume: 15, edition: March 2024, DOI
2023
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Engineering of Pseudomonas putida for accelerated co-utilization of glucose and cellobiose yields aerobic overproduction of pyruvate explained by an upgraded metabolic model
Metabolic Engineering, year: 2023, volume: 75, edition: January, DOI
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Mutualistic bacterial consortium for the co-utilisation of lignocellulosic disaccharides
Year: 2023, type: Appeared in Conference without Proceedings
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SEVA 4.0: an update of the Standard European Vector Architecture database for advanced analysis and programming of bacterial phenotypes
Nucleic Acids Research, year: 2023, volume: 51, edition: D1, DOI
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Unlocking the potential of inedible plant biomass using microbial engineering and synthetic biology
Year: 2023, type: Appeared in Conference without Proceedings
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Unlocking the potential of inedile plant biomass: From field to fueltank
Year: 2023, type: Appeared in Conference without Proceedings
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Upgrading Pseudomonas putida by systems metabolic engineering for biotechnological processing of lignocellulosic sugars
Year: 2023, type: Appeared in Conference without Proceedings
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Upgrading Pseudomonas putida by systems metabolic engineering for lignocellulose biotechnology
Year: 2023, type: Appeared in Conference without Proceedings
2022
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Engineering of Pseudomonas putida for fast co-utilization of glucose and cellobiose yields aerobic overproduction of pyruvate
Year: 2022, type: Appeared in Conference without Proceedings
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Polyhydroxyalkanoates synthesis by halophiles and thermophiles: towards sustainable production of microbial bioplastics
Biotechnology Advances, year: 2022, volume: 58, edition: September, DOI