Informace o projektu
Syntetické konsorcium kmenů Pseudomonas putida pro biodegradaci a ko-utilizaci (hemi)celulózových polymerů
- Kód projektu
- GA22-12505S
- Období řešení
- 1/2022 - 12/2024
- Investor / Programový rámec / typ projektu
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Grantová agentura ČR
- Standardní projekty
- Fakulta / Pracoviště MU
- Přírodovědecká fakulta
Pseudomonas putida KT2440 has emerged as a promising bacterial platform for lignocellulose biotechnology. It is well known for its abilities to degrade polymeric lignin, catabolise and even valorise resulting aromatic chemicals. Its biochemical network has been recently expanded toward utilisation of hemicellulosic pentose sugars and their co-assimilation with lignin aromatics and cellulosic glucose. These engineering endeavours proved the exceptional metabolic flexibility of P. putida. However, its foreseen potential to degrade and use cellulosic and hemicellulosic polymers and oligomers has not yet been explored. In this project, we aim to fill in this knowledge gap. We will adopt cutting-edge genetic engineering tools for the construction of a synthetic P. putida-based consortium capable of the parallel degradation of model (hemi)cellulosic polymers and co-utilisation of the gained oligomeric and monomeric hexose and pentose sugars. Once proven functional, the consortium will become a significant step toward consolidated bioprocessing of lignocellulosic residues by P. putida.
Cíle udržitelného rozvoje
Masarykova univerzita se hlásí k cílům udržitelného rozvoje OSN, jejichž záměrem je do roku 2030 zlepšit podmínky a kvalitu života na naší planetě.
Publikace
Počet publikací: 10
2024
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Synthetically-primed adaptation of Pseudomonas putida to a non-native substrate D-xylose
Nature Communications, rok: 2024, ročník: 15, vydání: March 2024, DOI
2023
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Engineering of Pseudomonas putida for accelerated co-utilization of glucose and cellobiose yields aerobic overproduction of pyruvate explained by an upgraded metabolic model
Metabolic Engineering, rok: 2023, ročník: 75, vydání: January, DOI
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Mutualistic bacterial consortium for the co-utilisation of lignocellulosic disaccharides
Rok: 2023, druh: Další prezentace na konferencích
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SEVA 4.0: an update of the Standard European Vector Architecture database for advanced analysis and programming of bacterial phenotypes
Nucleic Acids Research, rok: 2023, ročník: 51, vydání: D1, DOI
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Unlocking the potential of inedible plant biomass using microbial engineering and synthetic biology
Rok: 2023, druh: Další prezentace na konferencích
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Unlocking the potential of inedile plant biomass: From field to fueltank
Rok: 2023, druh: Další prezentace na konferencích
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Upgrading Pseudomonas putida by systems metabolic engineering for biotechnological processing of lignocellulosic sugars
Rok: 2023, druh: Další prezentace na konferencích
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Upgrading Pseudomonas putida by systems metabolic engineering for lignocellulose biotechnology
Rok: 2023, druh: Další prezentace na konferencích
2022
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Engineering of Pseudomonas putida for fast co-utilization of glucose and cellobiose yields aerobic overproduction of pyruvate
Rok: 2022, druh: Další prezentace na konferencích
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Polyhydroxyalkanoates synthesis by halophiles and thermophiles: towards sustainable production of microbial bioplastics
Biotechnology Advances, rok: 2022, ročník: 58, vydání: September, DOI