Project information
Epithelial Tissue Engineering: Cells and Protocols for Regenerative Medicine
(StemRepair)
- Project Identification
- 16-31501A (kod CEP: NV16-31501A)
- Project Period
- 4/2016 - 12/2019
- Investor / Pogramme / Project type
-
Ministry of Health of the CR
- Programme NV (2015 - 2022)
- MU Faculty or unit
- Faculty of Medicine
Cell therapies will only enable the treatment of diseases if an adequate number of therapeutic-grade cells are available. Thanks to their unlimited capacity for proliferation and differentiation, pluripotent embryonic stem cells (SCs) and their human-derived counterparts – induced pluripotent stem cells (iPSCs) – offer a means of meeting this requirement. Human iPSCs are particularly attractive, as they enable the preparation of autologous cells for a specific patient. The experiments included in this project will lead to the design and validation of techniques for the production of clinically relevant quantities of human progenitor cells of the vascular endothelium, pulmonary epithelium and smooth muscle cells, which will have a clear capacity to develop into mature, functional cells with therapeutic potential. This objective will be achieved by generating iPSCs from various types of human somatic cells and selecting the best ones based on their differentiation capacity and their safety in terms of genetic stability. These cells and the associated protocols will provide a source for the treatment of diseases characterised by abnormal vascularisation or lung epithelial function.
Publications
Total number of publications: 18
2024
-
Decellularization of Pig Lung to Yield Three-Dimensional Scaffold for Lung Tissue Engineering
3D Cell Culture. Methods in Molecular Biology, edition: Vyd. 2., year: 2024, number of pages: 13 s., DOI
2022
-
Expandable Lung Epithelium Differentiated from Human Embryonic Stem Cells
TISSUE ENGINEERING AND REGENERATIVE MEDICINE, year: 2022, volume: 19, edition: 5, DOI
2020
-
Endothelial Progenitor Cells Produced From Human Pluripotent Stem Cells by a Synergistic Combination of Cytokines, Small Compounds, and Serum-Free Medium
Frontiers in Cell and Developmental Biology, year: 2020, volume: 8, edition: 309, DOI
2019
-
HMGB2 is a negative regulator of telomerase activity in human embryonic stem and progenitor cells
FASEB Journal, year: 2019, volume: 33, edition: 12, DOI
-
Human Embryonic Stem Cells Acquire Responsiveness to TRAIL upon Exposure to Cisplatin
Stem Cells International, year: 2019, volume: 2019, edition: JAN 21 2019, DOI
-
In vitro model of alveolar capillary barrier
Year: 2019, type: Conference abstract
-
Novel synergistic culturing approach for efficient and robust differentiation of human pluripotent stem cells into endothelial progenitors.
Year: 2019, type: Conference abstract
-
Options for modeling the respiratory system: inserts, scaffolds and microfluidic chips
Drug Discovery Today, year: 2019, volume: 24, edition: 4, DOI
-
The Effect of Uncoated SPIONs on hiPSC-Differentiated Endothelial Cells
International Journal of Molecular Sciences, year: 2019, volume: 20, edition: 14, DOI
2018
-
Enhanced bioactivity of electrospun PCL and PLLA scaffolds blended with amino-phosphazene
Materials Letters, year: 2018, volume: 228, edition: OCT 1 2018, DOI