Project information
Postgenomic studies on Treponema pallidum, the syphilis spirochete

Information

This project doesn't include Faculty of Science. It includes Faculty of Medicine. Official project website can be found on muni.cz.

Project Identification

GA310/04/0021

Project Period

1/2004 - 12/2006

Investor / Pogramme / Project type

Czech Science Foundation

Standard Projects

MU Faculty or unit

Faculty of Medicine

Keywords

treponema pallidum, functional genomics, DNA microarray

The genome sequence of Treponema has been completed. Treponema pallidum subspecies pallidum is the causative agent of syphilis and is unculturable under in vitro conditions. This fact severly limits the use of standard genetic approaches to study this organism. We constructed the larga insert library of T. pallidum DNA in E. coli harborin 98,7 % of all T. pallidum genes on 19 individual clones. We constructed also DNA microarrays containing PCR products of all 1039 predicted T. pallidum ORFs. i) Methods of functional genomics will be used for gene function identifications. ii) Closely related T. paraluicuniculi is the etiologic agent of veneral spirochetosis of rabbits and is not infectious to humans. Genomic comparisons of both treponemes can provide evidence of genetic diversity that can be related to the different pathogenicity of these closely related bacteria. DNA microarray-based technology allows comparisons between these organism on both DNA (genome structure) and mRNA (expression profiling) levels. iii) The 19 canonical clones will be used to perform experiments on T. pallidum gene regulation within E. coli. Predicted T. pallidum regulators will be cloned on BAC-compatible expression of the cosmid-encoded T. pallidum genes in E.coli induced by the vector-encoded T. pallidum regulators will be examined. Similar regulation of a set of genes will be used for predictions of gene functions.

Publications

Total number of publications: 45

2006

Studium promotorů Treponema pallidum: charakterizace promotorů pro faktor sigma-54.

ČEJKOVÁ Darina MATĚJKOVÁ Petra ŠMAJS David

Article in Proceedings

X. Pracovní setkání biochemiků a molekulárních biologů. Sborník příspěvků., year: 2006

2005

Comparing genomes of pathogenic and non-pathogenic spirochetes: DNA-microarray hybridizations, whole genome fingerprinting and DNA sequencing.

ŠMAJS David MATĚJKOVÁ Petra STROUHAL Michal NORRIS S.J. WEINSTOCK G.M.

Conference abstract

Year: 2005, type: Conference abstract
Comparison of the genomes of pathogenic treponemes, Treponema pallidum ssp. pallidum, ssp. pertenue and Treponema paraluiscuniculi.

ŠMAJS David STROUHAL Michal MATĚJKOVÁ Petra NORRIS S.J. WEINSTOCK G.M.

Article in Proceedings

Clinical Microbiology and Infection, year: 2005
Genomics of Treponema.

WEINSTOCK G.M. ŠMAJS David MATĚJKOVÁ Petra NORRIS S.J. PALZKILL T. ALBERT T.J. SODERGREN E.

Conference abstract

Year: 2005, type: Conference abstract
Genomová amplifikace a restrikční mapování patogenních spirochet rodu Treponema.

STROUHAL Michal ŠMAJS David

Conference abstract

Year: 2005, type: Conference abstract
Genomová hybridizace kmenů T. pallidum Nichols a SS14 na oligonukleotidovém čipu

MATĚJKOVÁ Petra ŠMAJS David

Conference abstract

Year: 2005, type: Conference abstract
Identifikace regulátorů genové exprese u Treponema pallidum.

ČEJKOVÁ Darina ŠMAJS David

Article in Proceedings

Sborník příspěvků IX. Pracovního setkání biochemiků a molekulárních biologů, year: 2005
Komparativní genomika Treponema pallidum technikou genomové hybridizace na oligonukleotidovém čipu.

MATĚJKOVÁ Petra ŠMAJS David

Conference abstract

Year: 2005, type: Conference abstract
Mapování genomů patogenních spirochet.

STROUHAL Michal ŠMAJS David

Article in Proceedings

Sborník příspěvků IX. Pracovního setkání biochemiků a molekulárních biologů, year: 2005
Reproducibility of Comparative Genomics Sequencing in Treponema pallidum ssp. pallidum.

MATĚJKOVÁ Petra ŠMAJS David ALBERT T.J. NORRIS S.J. WEINSTOCK G.M.

Conference abstract

Year: 2005, type: Conference abstract