Project information
Transkription activation system for studying the relationship between cytokinin metabolism and action in Arabidopsis and tobacco
- Project Identification
- IAA5004001
- Project Period
- 1/2000 - 1/2002
- Investor / Pogramme / Project type
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Academy of Sciences of the Czech Republic
- Grants of distinctly investigative character focused on the sphere of research pursued at present particularly in the ASCR
- MU Faculty or unit
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Faculty of Science
- RNDr. Alena Kuderová, CSc.
- Cooperating Organization
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Institute of Biophysics
- Responsible person prof. RNDr. Břetislav Brzobohatý, CSc.
- Responsible person RNDr. Ivana Macháčková, CSc.
Cytokinins (CKs) are known to act in combination with other phytohormones to regulate diverse developmental processes in plants. However, knowledge of molecualr mechanism of CK action is limited. We will use a novel transcription activation system for regulated gene expression to create transgenic plants overproducing individually heterologous enzymes generating free CKs either by glucoside hydrolysis or by de novo synthesis in defined spatial and temporal patterns. We expect that CK release from glucosides will cause. in addition to common effects. several distrinct effects compared to de novo CK synthesis. Analysis of the distinct effects will contribute to understanding of complexity of CK metobolic conversions. This approach will lead to isolation of Arabidopsis lines displaying phenotypes corresponding to defined CK overproduction patterns. In future, the lines will be used, via isolation of suppresor mutations, to isolate genes involved in CK metabolism, and CK signal pathways.
Publications
Total number of publications: 19
2005
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pOp6/LhGR: a stringently regulated and highly responsive dexamethasone-inducible gene expression system for tobacco.
The Plant Journal, year: 2005, volume: 41, edition: 6
2004
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Ectopic overexpression of a Maize beta-glucosidase Disrupts Cytokinin Metabolism in Transgenic Tobacco.
Book of Abstracts, Plant Biology 2004, year: 2004
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Ectopic overexpression of a Maize beta-glucosidase Interferes with Cytokinin Homeostasis in Transgenic Tobacco.
18th IPGSA Conference, Book of Abstracts, year: 2004
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Overexpression of a Maize beta-glucosidase Disrupts Cytokinin Metabolism in Transgenic Tobacco
X. Days of Plant Physiology, Book of Abstracts, year: 2004
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Overexpression of a Maize beta-glucosidase Disrupts Cytokinin Metabolism in Transgenic Tobacco.
Acta Physiologiae Plantarum - Supplement 14th FESPB Congress Book of Abstracts, year: 2004
2003
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Dynamics of Endogenous Cytokinin Pools in Tobacco Seedlings: A Modelling Approach
Annals of Botany, year: 2003, volume: 91, edition: 5
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Ectopic overexpression of a Maize beta-glucosidase Disrupts Cytokinin Metabolism in Transgenic Tobacco
Book of Abstracts, Plant Physiology Conference of Ph.D. Students and Young Scientists 2003, year: 2003
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Ectopic Overexpression of a Maize beta-glucosidase may Disrupt Cytokinin Homeostasis in Transgenic Tobacco
Sborník Příspěvků VII. Pracovní Setkání Biochemiků a Molekulárních Biologů, year: 2003
2002
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Distinct responses to activation of a cytokinin biosynthesis gene, ipt, in tobacco and Arabidopsis seedling development.
Year: 2002, type: Conference abstract
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Inceased Ethylene Production Can Account for Some of the Phenotype Alterations Accompanying Activation of a Cytokinin Biosynthesis Gene, ipt, During Germination and Early Seedling Development in Tobacco
NATO Advanced Research Workshop on Biology and Biotechnology of the Plant Hormone Ethylene 2002, year: 2002