Project information
The Effects of Laser Beam Micromanipulation on the Cell Ultrastructure and Life
- Project Identification
- GA304/01/1560
- Project Period
- 1/2001 - 1/2003
- Investor / Pogramme / Project type
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Czech Science Foundation
- Standard Projects
- MU Faculty or unit
- Faculty of Medicine
- Cooperating Organization
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Institute of Scientific Instruments of the ASCR, v. v. i.
- Responsible person prof. RNDr. Pavel Zemánek, Ph.D.
The goal of the project is an investigation of the effects of irradiation by both continuos and pulsed laser light on the cytoskeleton and membranes of protozoa and human cells in the cell cultures. Optical tweezers designed and constructed in the framew ork of the previous GA CR project (no.202/96/1077) at the Inst.of Scientific Instruments of the Academy of Sciences, can be used with advantage for manipulation with cells and for transfer of organelles within the cell. The recognition of the extent if i njury will help to establish the time limit for laser intracellular micromanipulation. The effects of short laser pulses (5 ns) in different wavelengths (1064,532, 355, 266 nm) on the ultrastructure and moving activities will be studied using the pulsed Nd:YAG laser Minilite II. It can be expected that, in intracellular manipulations by optical tweezers, at least minimal local impairment will be produced due to released heat and the fine ultrastructural interactions in the cytoskeleton and biomembranes
Publications
Total number of publications: 13
2006
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Cytoskelet vegetativních stádií a cyst nálevníků Blepharisma
XIV. Cytoskeletální klub, year: 2006
2003
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Adaptive methods of cytoskeleton data analysis obtained by confocal microscopy
IX. Cytoskeletální klub, year: 2003
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Effects of low-power laser irradiation on cell proliferation
Scripta Medica, year: 2003, volume: 76, edition: 3
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Changes in cell proliferation after lowpower laser irradiation
Memory in living systems, year: 2003
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Manipulation stress effect on a cell
XXVI. Dny lékařské biofyziky. Sborník abstrakt, year: 2003
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Protective cysts of Blepharisma undulans undulans - a model for the study of damage produced by a laser beam
Memory in living systems, year: 2003
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Response of Infusorian cells to injury caused by a laser microbeam
Scripta Medica, year: 2003, volume: 76, edition: 4
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Therapeutical laser effects on model cell systems
Journal of Applied Biomedicine, year: 2003, volume: 1(Suppl.1), edition: Suppl.1
2002
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A manipulation stress effect of the therapeutic laser irradiation on actin cvytoskeleton
Proc. from Internat. Sci. Conf., Biologické dni, year: 2002
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Cytoskeleton response ot manipulation stress
Microscopy 2002, year: 2002