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Project information
Interactions defining physiological functions of Microtubule Associated Protein 2c at atomic resolution

Information

This project doesn't include Faculty of Science. It includes Central European Institute of Technology. Official project website can be found on muni.cz.
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Project Identification
GA20-12669S
Project Period
1/2020 - 12/2022
Investor / Pogramme / Project type
Czech Science Foundation
  • Standard Projects
MU Faculty or unit
Central European Institute of Technology
  • prof. Mgr. Lukáš Žídek, Ph.D.

Microtubule associated protein 2c (MAP2c) is a key factor regulating microtubule dynamics in
developing brain neurons, and an example of an intrinsically disordered proteins with an
important physiological function and detectable structure-function relationship. We propose to
study interactions of MAP2c defining and controlling its biological function. We focus on two
regions in the N-terminal half of MAP2c sequence that bind molecules not interacting with the
homologous protein Tau and contain structural motifs unique for MAP2c and not found in Tau.
We will study interactions with steroids, regulatory subunit of kinase PKA, plectin, and evaluate
effects of phosphorylation and interactions with regulatory 14-3-3 proteins. We also propose to
study impact of the interactions on microtubule binding and address biological relevance of the
in vitro results by recording NMR spectra at near-to-native conditions (in cells and/or cell
lysates) and performing cryo-electron tomography on monolayered neurons to observed
interactions of cytoskeleton components regulated by MAP2c binding in situ.

Sustainable Development Goals

Masaryk University is committed to the UN Sustainable Development Goals, which aim to improve the conditions and quality of life on our planet by 2030.

Sustainable Development Goal No. 3 – Good health and well-being Sustainable Development Goal No. 4 – Quality education

Publications

Total number of publications: 4

2025

2024

2022

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