Project information
Structure and DNA delivery mechanism of gene transfer agent
(GTA)
- Project Identification
- MUNI/E/0530/2019
- Project Period
- 6/2019 - 11/2020
- Investor / Pogramme / Project type
-
Masaryk University
- Grant Agency of Masaryk University
- MU Faculty or unit
-
Central European Institute of Technology
- Mgr. Pavol Bárdy, Ph.D.
- prof. RNDr. Jiří Doškař, CSc.
- Ing. Tibor Füzik, Ph.D.
- Mgr. Dominik Hrebík, Ph.D.
- prof. RNDr. Roman Pantůček, Ph.D.
- doc. Mgr. Pavel Plevka, Ph.D.
Alphaproteobacterial gene transfer agent (GTA) represents the most sophisticated system for horizontal gene transfer in prokaryotes discovered to date. Here, we present the near-atomic structure of Rhodobacter capsulatus GTA, first GTA discovered in nature, determined by cryo-EM. Furthermore, we describe the mechanism how GTA particles transfer DNA into the recipient cells. Our results show an evolutionary linkage between families of tailed phages and bring possibilities of controlling the gene transfer in ocean environments, where alphaproteobacteria prevail.
Sustainable Development Goals
Masaryk University is committed to the UN Sustainable Development Goals, which aim to improve the conditions and quality of life on our planet by 2030.
Publications
Total number of publications: 1
2020
-
Structure and mechanism of DNA delivery of a gene transfer agent
Nature Communications, year: 2020, volume: 11, edition: 1, DOI