Project information
Development of a new typing system for the causative agent of syphilis, Treponema pallidum subsp. pallidum, focusing on proteomic differences
- Project Identification
- 17-31333A (kod CEP: NV17-31333A)
- Project Period
- 4/2017 - 12/2020
- Investor / Pogramme / Project type
-
Ministry of Health of the CR
- Programme NV (2015 - 2022)
- MU Faculty or unit
- Faculty of Medicine
- Cooperating Organization
-
General Teaching Hospital Praha
- Responsible person doc. MUDr. Ivana Kuklová, CSc.
- Responsible person MUDr. Hana Zákoucká
Treponema pallidum subsp. pallidum (TPA) is the causative agent of the sexually transmitted disease syphilis. Globally, over 10 million new cases of syphilis are diagnosed each year. With the advancement of genomics, two molecular typing systems have been developed to characterise clinical TPA strains; however, their typing efficiency and discriminatory power vary considerably. The aim of this project is to develop a new, highly reliable and effective molecular typing system, focusing on sequences encoding proteomic differences between TPA strains, which may underlie varying clinical manifestations. The discriminatory power of the typing system will be tested on genetically distinct clinical isolates and samples from various locations around the world. Knowledge of the genetic basis of current clinical TPA strains is also crucial in terms of ongoing efforts to develop a vaccine against syphilis. The application of the new typing system to clinical strains from the Czech Republic will provide information on the diversity and prevalence of individual TPA genotypes within our country. The molecular data will be correlated with available epidemiological data.
Publications
Total number of publications: 15
2018
-
Genetics of human and animal uncultivable treponemal pathogens
Infection, Genetics and Evolution, year: 2018, volume: 61, edition: JUL 2018, DOI
-
Molecular characterization of Treponema pallidum subsp pallidum in Switzerland and France with a new multilocus sequence typing scheme
Plos one, year: 2018, volume: 13, edition: 7, DOI
-
Multi-locus sequence typing of Treponema pallidum subsp pallidum present in clinical samples from France: Infecting treponemes are genetically diverse and belong to 18 allelic profiles
Plos one, year: 2018, volume: 13, edition: 7, DOI
-
Sequence Variation of Rare Outer Membrane Protein beta-Barrel Domains in Clinical Strains Provides Insights into the Evolution of Treponema pallidum subsp. pallidum, the Syphilis Spirochete
MBIO, year: 2018, volume: 9, edition: 3, DOI
-
Sequencing of Treponema pallidum subsp pallidum from isolate UZ1974 using Anti-Treponemal Antibodies Enrichment: First complete whole genome sequence obtained directly from human clinical material
Plos one, year: 2018, volume: 13, edition: 8, DOI