Project information
Design of fluorescence-based analytical tool for quantification of the protein-protein interaction within the 14-3-3 family
- Project Identification
- 7AMB16DE005
- Project Period
- 1/2016 - 12/2017
- Investor / Pogramme / Project type
-
Ministry of Education, Youth and Sports of the CR
- Mobility Activity
- MU Faculty or unit
- Central European Institute of Technology
The 14-3 -3 protein family plays a central role in signal transduction by recognizing phosphorylated serine/threonine sequence motifs in client proteins and by modulating their function. These sequence motifs are bound by the two binding sites of a 14-3-3 protein, which are only present in a dimeric structure. Phosphorylation of S58 within the 14-3-3 homodimer triggers dissociation of the monomeric subunits, which may impair their function. Thus, while having appreciated importance of the integrity of dimeric structure for a proper biological function, no quantitative analytical tools for dimer-monomer equilibrium have been developed until now.
In the current proposal, an international, interdisciplinary team of researchers suggests to develop an efficient fluorescence-based approach for studying equilibrium and kinetics of quaternary structure formation of members of the 14-3-3 families. The 14-3-3zeta protein will be purified, specifically labeled by fluorescent dyes and used for FRET experiments, microscale thermophoresis, NMR as well as being investigated by computational methods.
Publications
Total number of publications: 1
2022
-
Quantitation of Human 14-3-3ζ Dimerization and the Effect of Phosphorylation on Dimer-monomer Equilibria
Journal of Molecular Biology, year: 2022, volume: 434, edition: 7, DOI