Project information
Mechanism of plant resistance against ROS induced in photosynthetic apparatus by a photoinhibitory stress
- Project Identification
- GA522/06/0979
- Project Period
- 1/2006 - 12/2008
- Investor / Pogramme / Project type
-
Czech Science Foundation
- Standard Projects
- MU Faculty or unit
- Faculty of Science
- Keywords
- reactive oxygen species, photoinhibition, chlorophyll fluorescence, D1 protein, stress proteins
The proposed project is focused on the principles of plant resistance against the effects of high light-induced reactive oxygen species (ROS) in photosynthetic apparatus. Two model plants were selected to study photoinhibition under a variety of light and temperature conditions: lichenized green alga Trebouxia and Arabidopsis thaliana. Detection of photoinhibition and photodamage to PS II will be done by fluorometric methods (fast Chl induction curve, quenching analysis) and molecular biology methods (D1 protein turnover and ELIPs detection). Photoprotective mechanisms, xanthophyll cycle pigments and antioxidative substrate (glutathion) activated during the early stages of photoinhibition will be evaluated biochemically. Actual level of ROS will be evaluated by specific sensor molecules monitored during and after photoinhibition by the means of fluorescence imaging technique
Publications
Total number of publications: 14
2009
-
Does amount of light harvesting complexes play a role in the sensitivity of DCMU-treated Arabidopsis thaliana to high light stress?
Year: 2009, type: Conference abstract
2008
-
Analysis of <i>A. thaliana</i> resistence to high light stress and reactive oxygen species formation using biophysical methods
Year: 2008, type: Conference abstract
-
Duration of irradiation rather than quantity and frequency of high irradiance inhibits photosynthetic processes in the lichen <i>Lasallia pustulata<i>
Photosynthetica, year: 2008, volume: 46, edition: 2
-
Chlorophyll fluorescence: A wonderful tool to study plant physiology and plant stress
Plant Cell Compartments - Selected Topics, year: 2008, number of pages: 64 s.
-
Irradiance prior to and during desiccation improves the tolerance to excess irradiance in the desiccated state of the old forest lichen <i>Lobaria pulmonaria<i>
Photosynthetica, year: 2008, volume: 46, edition: 2
-
Responses of PS II to high light in LHC-deficient Arabidopsis thaliana mutants detected by two chlorophyll fluorescence techniques
Physiologia Plantarum. Special Issue: Redox Signal Integration. FESPB 2008 Abstracts, year: 2008
-
Short-term responses of photoprotective mechanisms to artificial photoinhibition in Arabidopsis thaliana
International Conference of PhD Students on Experimental Botany: Book of abstracts, year: 2008
2007
-
Glutathione and zeaxanthin formation during high light stress in foliose lichens
Plant, Soil and Environment, year: 2007, volume: 53/2007, edition: 8
-
High light induced ROS and their effects on photoinhibition in <i>Arabidopsis thaliana</i> mutants with reduced amount of light harvesting complexes
12th Congress of European Society for Photobiology, Book of abstracts, year: 2007
-
Množství světlosběrných komplexů ovlivňuje stupeň fotoinhibice fotosyntézy u LHC mutantů Arabidopsis thaliana
Sborník Abstraktů. Konference experimentální biologie rostlin, 11. Dny rostlinné fyziologie, year: 2007