Project information
Changes in the structure of interphase nucleai of human leukemic cell lines after treatment with differentiating agents and gama irradiation
- Project Identification
- GA202/98/P253
- Project Period
- 1/1999 - 1/2001
- Investor / Pogramme / Project type
-
Czech Science Foundation
- Standard Projects
- MU Faculty or unit
-
Faculty of Informatics
- Keywords
- later
- Cooperating Organization
-
Institute of Biophysics
- Responsible person prof. RNDr. Eva Bártová, Ph.D., DSc.
The main objective of the proposed grant is the research of the changes of the nuclear chromatin structure in the human leukemic myeloid cell lines after the treatment with differentiating agents and after gamma-irradiation. Changes of abl, bcr and c-myc gene topology in cell nuclei will be detected. Translocation t(9,22);(q34,q11) which leads to chimeric bcr-abl gene (Philadelphia chromosome)that plays an important role in the pathogenesis of chronic myelogenous leukemia (CML) will be studied. Fluoresc ence in situ hybridisation (FISH) combined with the image analysis will be used. Changes in the structure of interphase nuclei will be related to the cell cycle profile and to the stages of cell maturation. In the frame of this project, the reverse trans cription polymerase chain reaction (RT-PCR) for c-myc and abl-bcr gene expression will be applied. C-myc protooncogene plays an essential role in the regulation of G1 phase of the cell cycle and is important in the initiation of myeloid differentiation a
Publications
Total number of publications: 4
2001
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Higher-order chromatin structure of human granulocytes
Chromosoma, year: 2001, volume: 110, edition: 5
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Spatial distribution of selected genetic loci in nuclei of human leukemia cells after irradiation
Radiation Research, year: 2001, volume: 155, edition: 2
2000
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Nuclear topography of the c-myc gene in human leukemic cells
Gene, year: 2000, volume: 244, edition: 1
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The influence of the cell cycle, differentiation and irradiation on the nuclear location of the ABL, BCR and C-MYC genes in human leukemic cells
Leukemia Research, year: 2000, volume: 24, edition: 3