Informace o projektu
Regulace rostlinné mei?zy a vývoj technologií k její manipulaci
- Kód projektu
- CZ.02.1.01/0.0/0.0/15_003/0000479 (kod CEP: EF15_003/0000479)
- Období řešení
- 1/2017 - 10/2022
- Investor / Programový rámec / typ projektu
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Ministerstvo školství, mládeže a tělovýchovy ČR
- OP Výzkum, vývoj a vzdělávání (OP VVV)
- PO 1 Posilování kapacit pro kvalitní výzkum
- Fakulta / Pracoviště MU
- Středoevropský technologický institut
Meiosis lies at the heart of sexual reproduction, the most successful reproductive strategy creating new genetic combinations every generation. This project aims at understanding the mechanisms that define meiotic division and gamete differentiation in plants; part of the effort will be establishing technologies allowing in vitro differentiation of male gametes, live imaging of meiosis and the manipulation of meiotic processes. The approaches will include microfluidics for in vitro cultivation of reproductive tissues coupled with time-laps microscopy (anther on chip technology), next generation sequencing and bioinformatics for identification of new meiotic regulators, CRISPR/Cas9 genome editing and development of techniques for the delivery of biologically active compounds into meiotic. This project well complements ongoing research activities at CEITEC and will strengthen its excellence at multiple levels: 1) It brings unique technology and instrumentation, which will yield important and highly visible discoveries. 2) It combines different expertise and contributes to the multidisciplinarity of the research centre. 3) It has applied potential in plant breeding. 4) It will facilitate establishing close ties to the Vienna Biocenter and promote cross-border collaboration with this prestigious research cluster.
Cíle udržitelného rozvoje
Masarykova univerzita se hlásí k cílům udržitelného rozvoje OSN, jejichž záměrem je do roku 2030 zlepšit podmínky a kvalitu života na naší planetě.
Publikace
Počet publikací: 17
2023
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A complex role of Arabidopsis CDKD;3 in meiotic progression and cytokinesis
Plant Direct, rok: 2023, ročník: 7, vydání: 3, DOI
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High temperature increases centromere-mediated genome elimination frequency and enhances haploid induction in Arabidopsis
Plant Communications, rok: 2023, ročník: 4, vydání: 3, DOI
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The biological functions of nonsense-mediated mRNA decay in plants: RNA quality control and beyond
Biochemical Society Transactions, rok: 2023, ročník: 51, vydání: 1, DOI
2022
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Application of Chemical Inhibitors in Live Cell Imaging of Plant Meiosis Using Light Sheet Fluorescence Microscopy
Plant Gametogenesis. Methods in Molecular Biology, vol 2484, rok: 2022, počet stran: 13 s.
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B1-type cyclins control microtubule organization during cell division in Arabidopsis
EMBO reports, rok: 2022, ročník: 23, vydání: 1, DOI
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Meiotic exit in Arabidopsis is driven by P-body-mediated inhibition of translation
Science, rok: 2022, ročník: 377, vydání: 6606, DOI
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Transcriptional and post-transcriptional regulation of young genes in plants
BMC Biology, rok: 2022, ročník: 20, vydání: 1, DOI
2021
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A CENH3 mutation promotes meiotic exit and restores fertility in SMG7-deficient Arabidopsis
PLoS Genetics, rok: 2021, ročník: 17, vydání: 9, DOI
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A hypomorphic allele of telomerase uncovers the minimal functional length of telomeres in Arabidopsis
Genetics, rok: 2021, ročník: 219, vydání: 2, DOI
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High-Throughput Protein-Nucleic Acid Interaction Assay Based on Protein-Induced Fluorescence Enhancement
RNA REMODELING PROTEINS, rok: 2021, počet stran: 9 s.